The King Lab

Cilia and flagella are highly complex organelles with >500 different protein components and our laboratory is interested in the general mechanisms by which these organelles are assembled and how motility is achieved. We have focused our attention on the dynein arms that power these organelles and study how these massive molecular motors are assembled and organized, the mechanisms by which force production is achieved and the pathways by which the motors are regulated.

The laboratory is located on the third floor of the University of Connecticut Health Center and is well equipped for Chlamydomonas culture, biochemistry and microscopy. We also have access to various core facilities including:

• Electron microscopy
• The Center for Cell Analysis and Modeling
• The biophysical core which contains a CD spectrophotometer, both isothermal titration and differential scanning calorimeters, a mass spectrometer, and HPLC equipment
• Gregory P. Mullen Structural Biology facility that currently houses 400, 500 and 600 MHz NMR spectrometers, and a computational facility (an NIH-funded 800 MHz spectrometer will be installed in the near future)
• An X-ray generator which is located in the laboratory of Dr Bing Hao.